課程資訊
課程名稱
 電顯技術在植病之應用
Electronmicroscopy Application in Phytopathology 
開課學期
 101-2 
授課對象
 生物資源暨農學院  植物病理與微生物學系  
授課教師
 曾顯雄 
課號
 PPM5016 
課程識別碼
 633 U0650 
班次
  
學分
 3 
全/半年
 半年 
必/選修
 選修 
上課時間
 星期一5,6,7,8,9(12:20~17:20) 
上課地點
 總一館308 
備註
 與鄭穹翔合開
總人數上限：8人 
Ceiba 課程網頁
 http://ceiba.ntu.edu.tw/1012electapplication 
課程簡介影片
 
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課程概述

本課程之主要授課內容係在講解掃描式暨穿透式電子顯微鏡之研發歷程、機件組合、操作流程、材料製備、低溫冷凍、X光能量元素分析、超薄切片、細胞免疫化學等，以及其在植物病理學與微生物學之應用。 

課程目標
 . 
課程要求
  
預期每週課後學習時數
  
Office Hours
  
指定閱讀
  
參考書目
 Reference:
1.陳家全、李家維、楊瑞森•1991•生物電子顯微鏡學•國科會儀器叢書四•
2.彭紹恩、胡景雯、陳啟祥•2010•圖解穿透式電子顯微鏡術•海生館•
3.Aldrich, H. C. and Todd, W. J. 1986. Ultra-structure Techniques for Microorganisms. Plenum Press. New York and London. 533pp.
4.Goldstein, J. I., D. E. Newbury, P. Echlin, D. C. Joy, C. E. Lyman, E. Lifshin, L. Sawyer and J. R. Michael. 2003. Scanning Electron Microscopy and X-Ray Microanalysis. Springer, New York. 689pp.
5.Kuo, J. 2007. Electron Microscopy: Methods and Protocols. Humana Press.
6.Hayat, M. A. 1978. Introduction to Biological Scanning Electron Microscopy. University. Park Press, Baltimore, Maryland. 323pp.
7.Wischnitzer, S. 1989. Introduction to Electron microscopy. 3rd ed. Pergamon Press, 405pp.
 
評量方式
(僅供參考)
  
No.
項目
百分比
說明
1. 
 期中考 
35% 
  
2. 
 期末考 
35% 
  
3. 
 期末報告 
30% 
 SEM、TEM部分，期末繳交閱讀最近三年科學期刊中有關SEM、TEM之研究論文報告，暨上台報告此讀書報告之內容，每位同學25分鐘，內含5分鐘之討論、交換心得。報告內容不得拷貝任何專家學者所撰寫之報告、回顧（review）等資料，否則不予計分。 
 
課程進度
週次
日期
單元主題
第1週
 2/18  S. S. Tzean.
Lecture-Instrumentation of scanning electron microscopy.
Laboratory Exercise-Configuration of SEM. 
第2週
 2/25  S. S. Tzean.
Lecture-Operation of SEM and microphotography.
Laboratory Exercise-SEM: operation and photography  
第3週
 3/04  S. S. Tzean. Lecture-Preparation of specimen for SEM. Laboratory Exercise-Specimen preparation: fixation and dehydration.  
第4週
 3/11  S. S. Tzean. Lecture-Preparation of specimen for Cryo-SEM. Laboratory Exercise-specimen preparation: critical point drying and coating. 
第5週
 3/18  S. S. Tzean. Lecture-Preparation of specimen for SEM EDAX. Laboratory Exercise-SEM: specimen observation  
第6週
 3/25  S. S. Tzean. Lecture-SEM-Immunocytochemistry. Laboratory Exercise-SEM: specimen observation 
第7週
 4/01  S. S. Tzean. Lecture-Application of electron microscopy in plant pathology. Laboratory Exercise-Cryo-SEM: configuration and illustration. 
第8週
 4/08  S. S. Tzean. Lecture-Student’s presentation: recent advancement in SEM technology. Laboratory Exercise-SEM EDAX: structure, operation and elements analysis 
第9週
 4/15  S. S. Tzean. Lecture-Student’s presentation: recent advancement in SEM technology. Laboratory Exercise-Presentation: recent advancement in SEM technology 
第10週
 4/22  C. H. Cheng. Lecture-History and application of transmission electron microscopy. Laboratory Exercise-Instrumentation and operation of TEM 
第11週
 4/29  C. H. Cheng. Lecture-Application of TEM in biology.Factors affecting the quality of fixation. Laboratory Exercise-Specimen preparation, sectioning, and staining for TEM 
第12週
 5/06  C. H. Cheng. Lecture-Preparation of specimen for TEM Immuno-electron microscopy. Laboratory Exercise-Specimen preparation, sectioning, and staining for immuno-electron microscopy
 
第13週
 5/13  C. H. Cheng. Lecture-Preparation of specimen for TEM. Laboratory Exercise-Observations of specimen and recording 
第14週
 5/20  C. H. Cheng. Lecture-Recent advancement in TEM technology for biological study. Laboratory Exercise-Observations of specimen and recording 
第15週
 5/27  C. H. Cheng. Lecture-Students’presentation: recent advancement in TEM technology. Laboratory Exercise-Observations of specimen and recording 
第16週
 6/03  C. H. Cheng. Lecture-Students’ presentation: recent advancement in TEM technology. Laboratory Exercise-3D-TEM  
第17週
 6/10  Lecture-SEM(Test) Laboratory Exercise-Test for SEM operation 
第18週
 6/17  Lecture-TEM(Test) Laboratory Exercise-Test for TEM operation